An established massage technique for semen collection in budgerigars, cockatiels, and monk parakeets was applied to 40 different birds of 26 different psittacine species in a period of 4 weeks. Semen collection was successful in 8 individual birds (Aratinga species, Tanygnathus species, Guarouba guarouba, and Pionus chalcopterus). Large parrot species (Ara ambigua, Cacatua leadbeateri, Amazona species, and Eclectus species) were difficult to handle and did not relax during the application of the massage technique. Semen was evaluated for volume, color, pH, spermatozoa concentration, total spermatozoa number, spermatozoa motility, and fraction of living spermatozoa. Additionally, spermatozoa were examined for pathomorphologic abnormalities.